MULTICHANNEL SYSTEM-MULTIELECTRODE ARRAY 200µm/30µm
Extracellular Recording with Microelectrode Arrays:
A microelectrode array (MEA) is an arrangement of typically 60 electrodes allowing the targeting of several sites in parallel for extracellular recording and stimulation.
Cell lines or primary cell preparations are cultivated directly on the MEA. Freshly prepared slices can be used for acute recordings, or can be cultivated as organotypic cultures (OTC) on the MEA.
Recorded signals are amplified by a filter amplifier and sent to the data acquisition computer. All MEAs (except EcoFlexor FlexMEAs) are only for use with MEA2100- or USB-MEA-Systems for extracellular recording from Multi Channel Systems
MCS GmbH. FlexMEAs may be used with components of ME2100-System and USB-ME-Systems from Multi Channel Systems
MCS GmbH. EcoFlex- and FlexMEAs are designed for use in in vitro or in vivo studies. Please see setup manuals “Setup (USB-) MEA-Systems and (USB-) ME-Systems” for more information.
DESCRIPTION OF 60ThinMEA
Thin MEA: 60ThinMEA
60ThinMEA200µm/30µmiR-ITO
⦁ 60ThinMEAs are only 180 µm "thick"
⦁ Ideally suited for high-resolution imaging.
⦁ 60ThinMEAs are like standard MEAs, but the electrodes are embedded in a very thin and delicate glass substrate on a robust ceramic carrier.
⦁ The thin glass allows the use of oil immersion objectives with a high numerical aperture.
⦁ They dont have wells and their transparenty.
⦁ There is no well in this type of MEA.
The electrodes of the 60-6wellMEA are
⦁ From titanium nitride (TiN)
⦁ The isolation is made up of Silicon nitride (SiN).
⦁ Contact pads are from titanium nitride (TiN)
⦁ Tracks are from titanium (Ti).
⦁ The diameter of the electrodes is 30 µm
⦁ The distance from center to center is 200 µm.
APPLICATION
⦁ 60ThinMEAs are heat-stabilized and can be autoclaved.
⦁ They can also be coated with different procedures for cell and tissuecultures.
⦁ They should be handled with great care because of the thin and delicate recording area.
60 Standard microelectrode array(MEA) Layout
- Base material: Glass
- Electrode material: TiN (Titanium nitride)
- Electrode height: planar
- Track material: ITO (Indium tin oxide) or Ti (Titanium)
- Contact pads: ITO (Indium tin oxide) or TiN (Titanium nitride)
- Electrode diameter; 30 μm or 10 μm
- Interelectrode distance (center to center): 100 µm to 200 μm
- Isolation material: Silicon nitride 500 nm (PEVCD)
- Electrode impedance; < 100 kW for 30 µm electrodes, 250 - 400 kW for 10 μm electrodes
- Electrode layout grid: 8 x 8
- Number of recording electrodes: 59
- Number of reference electrodes: 1
- Software: MC_Rack MC_Rack: 2 dim. (MEA) or Configuration
- Software: Multi Channel Suite Multi Channel Experimenter: linear.
MEA Design and Production
A standard MEA biosensor has a square recording area of 700 µm to 5 mm length. In this area, 60 electrodes are aligned in an 8 x 8 grid with interelectrode distances of 100, 200, or 500 µm. Planar TiN (titanium nitride) electrodes are available in sizes of 10, 20, and 30 µm, and three-dimensional TiN electrodes have a diameter of > 12 µm at the base with a very fine tip. Standard
MEAs are useful for a wide variety of applications. Different geometries match the anatomical properties of the preparation.
Most MEAs are available with a substrate-integrated reference electrode replacing the silver pellet in the bath. All electrodes can either be used for recording or for stimulation.
60MEA200/30iR-Ti, 60MEA200/30iR-ITO, 60MEA200/10iR-Ti, 60MEA100/10iR-TI, 60MEA500/30iR-Ti, 60MEA500/30iR-ITO,
60MEA500/10iR-Ti.
The flat, round electrodes are made of titanium nitride (TiN). MEAs with TiN electrodes are very stable. Therefore, the MEAscan be reused several times and are perfect for long-time experiments (up to several weeks and even months). The electrode
impedance ranges between < 100 kΩ for 30 µm electrodes and 250 to 400 kΩ for 10 µm electrodes, depending on the electrode
diameter. Generally, the smaller the electrode, the higher is the impedance.
Tracks are made of titanium (Ti) and contact pads are made of titanium nitride (TiN) or indium tin oxide (ITO); insulation
material is silicon nitride. ITO contact pads and tracks are transparent, for a perfect view of the specimen under the microscope.
Standard microelectrode array have
- TiN electrodes
- SiN isolator
- Contact pads and tracks opaque (TiN)
- With 1 internal reference electrode
- Electrode grid 8x8
- 59 recording electrodes
- Electrode spacing 200 µm
- Electrode diameter 30 µm
There are various applications for MEAs in the fields of neurobiology or cardiac electrophysiology.
Typical neurobiological applications are:
- Ion channel screening
-Drug testing
-Safety pharmacology studies
-Current source density analysis
-Paired-pulse facilitation (PPF)
-long term potentiation (LTP) and depression (LTD)
-I / O relationship of evoked response
-Circadian rhythm
-Neuro-regeneration
-Developmental biology
-Microencephalograms (EEG) and microelectroretinograms (ERG).
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