(HSG-MEA-5C)ALA HARP SLICE GRID HSD-5 FOR MEA SYSTEM

ALA HARP SLICE GRID HSD-5 FOR MEA SYSTEM

Electrophysiology and imaging experiments with submerged slices won’t work if the slices are drifting during perfusion. Securing slices with “homemade” grids requires spending time gluing bits of wire and nylon together and testing them on valuable preparations. Using ALA’s HARP SLICE GRIDS will provide more consistent results and more time to do experiments. ALA’s HARP SLICE GRIDS are constructed with durable and inert materials like glass-coated steel rings and polyimide-coated silica fibers or nylon and they are available in suitable configurations for most preparations. They are designed for busy researchers who want to focus their efforts on running experiments, not setting them up.

-ALA’s MEA HARP SLICE GRIDS for MEA system are constructed with durable and inert glass-coated steel rings with mesh that are available in suitable configurations for most preparations.
-The mesh lies both horizontally and vertically across the preparation forming squares of approximately 1mm2.
-This is the preferred type of grid when contact between the preparation and substrate electrodes is critical, such as during recording with multielectrode array systems.
- They are excellent when combining multielectrode array recording, such as the MEA60 system, with conventional recording and stimulation methods.
-Mesh on certain configurations made of nylon or polyester.
-White nylon available for certain configurations. See Specs for details below / on download.

ALA’s Harp Slice Grids hold down brain slices during electrophysiology experiments. They are available in various sizes, shapes and weights.

HSG-MEA-5A
-Dimensions: 9.5 mm ID x 12 mm OD x 0.5 mm thick
-Weigh 0.2 g
-Pack of 5 RING
-String: 50-65 µm OD
- String spacing about 1 mm
-Glass-coated steel attachment
-Rings are rigid, inert, and dense
-Polyimide-coated silica capillary tubing are also inert, long-lasting, rigid, and are
spaced widely to allow patch-pipettepositioning
-Tightly-woven nylon mesh are spaced narrowly and hold slices securely with
out impeding superfusate access.

HSG-MEA-5AD
-Dimensions: 9.5 mm ID x 12 mm OD x 1.0 mm thick
-Weigh 0.4 g
-Pack of 5 RING
-String: 50-65 µm OD
- String spacing about 1 mm
-Glass-coated steel attachment
-Rings are rigid, inert, and dense
-Polyimide-coated silica capillary tubing are also inert, long-lasting, rigid, and are
spaced widely to allow patch-pipettepositioning
-Tightly-woven nylon mesh are spaced narrowly and hold slices securely with
out impeding superfusate access

HSG-MEA-5B
-Dimensions: 9.5 mm ID x 12.75 mm OD x 0.83 mm thick
-Weigh 0.4 g
-Pack of 5 RING
-String: 50-65 µm OD
-String spacing about 1 mm
-Glass-coated steel attachment
-Rings are rigid, inert, and dense
-Polyimide-coated silica capillary tubing are also inert, long-lasting, rigid, and are
spaced widely to allow patch-pipettepositioning
-Tightly-woven nylon mesh are spaced narrowly and hold slices securely with
out impeding superfusate access.

HSG-MEA-5BD
-Dimensions: 9.5 mm ID x 12.75 mm OD x 1.64 mm thick
-Weigh 0.8 g
-Pack of 5 RING
-String: 50-65 µm OD
-String spacing about 1 mm
-Hold down slices (slice anchors) for submerged experiments
-Nylon strings (standard) or mesh (for MEA)
-Chemically inert: glass-coated steel rings
-Polyester mesh available.

HSG-MEA-5C
-Dimensions: 6.4 mm ID x 8 mm OD x 0.5 mm thick
-Weigh 0.05 g
-Pack of 5 RING
-Glass-coated steel attachment
-String: 50-65 µm OD
-String spacing about 1 mm
-Rings are rigid, inert, and dense
-Polyimide-coated silica capillary tubing are also inert, long-lasting, rigid, and are
spaced widely to allow patch-pipettepositioning
-Tightly-woven nylon mesh are spaced narrowly and hold slices securely with
out impeding superfusate access.

HSG-MEA-5CD
-Dimension; 6.4 mm ID x 8 mm OD x 1.0 mm thick.
-Weigh 0.05 g
-Pack of 5 RING
-String: 50-65 µm OD
-String spacing about 1 mm
-Hold down slices (slice anchors) for submerged experiments
-Nylon strings (standard) or mesh (for MEA)
-Chemically inert: glass-coated steel rings
-Polyester mesh available.

Harp Slice Grids are available in two basic types:
1. The first type uses
-Polyimide coated silica fibers as "strings" that lie across the preparation.
-The thinness of the "strings" (~50-65µm OD) combined with relatively wide spacing (about 1mm) facilitates electrode or pipette access from above the preparation.
- These are ideal for classical electrophysiological recordings with slices in submerged or
interface chambers.

1 mm silica fiber spacing
HSG-5A 9.5 mm ID x 12 mm OD x 0.5 mm thick; 0.2 g – 5 pack
HSG-5AD 9.5 mm ID x 12 mm OD x 1.0 mm thick and 0.4 g – 5 pack
HSG-5B 9.5 mm ID x 12.75 mm OD x 0.83 mm thick; 0.4 g – 5 pack
HSG-5BD 9.5 mm ID x 12.75 mm OD x 1.64 mm thick and 0.8 g – 5 pack
HSG-5C 6.4 mm ID x 8 mm OD x 0.5 mm thick; 0.05 g – 5 pack
HSG-5CD 6.4 mm ID x 8 mm OD x 1.0 mm thick and 0.1 g – 5 pack
HSG-5D 6.0 x 3.3 mm ID x 12.7 mm OD x 0.54 mm thick; 0.18 g – 5 pack – oval interior
HSG-5DD 6.0 x 3.3 mm ID x 12.7 mm OD x 1.07 mm thick and 0.35 g – 5 pack- oval interior
HSG-5E 4.8 x 2.5 mm ID x 9.5 mm OD x 0.5 mm thick; 0.1 g – 5 pack – oval interior
HSG-5ED 4.8 x 2.5 mm ID x 9.5 mm OD x .85 mm thick and 0.2 g – 5 pack – oval interior
HSG-5F 10.0 mm ID x 13.8 mm OD x 0.8 mm thick; 0.18 g – 5 pack – easy pick and place
HSG-5F 10.0 mm ID x 13.8 mm OD x 1.8 mm thick and 0.33 g – 5 pack – easy pick and place

2.The second type employs
- Nylon mesh in which the "strings" lay both horizontally and vertically across the preparation, forming squares of approximately 1 square mm.
-This is the preferred type when contact between the preparation and substrate electrodes is
critical, such as during recording with multielectrode array systems. Also they are excellent when combining multielectrode array recording, such as the MEA60 system, with conventional recording and stimulation methods.

We provide this type of ALA Harp slice Grid.
ALA Harp slice grid for MEA are; Nylon mesh type with strings crossing makes spacing 1 mm²
HSG-MEA-5A 9.5 mm ID x 12 mm OD x 0.5 mm thick; 0.2 g – 5 pack
HSG-MEA-5AD 9.5 mm ID x 12 mm OD x 1.0 mm thick and 0.4 g – 5 pack
HSG-MEA-5B 9.5 mm ID x 12.75 mm OD x 0.83 mm thick; 0.4 g – 5 pack
HSG-MEA-5BD 9.5 mm ID x 12.75 mm OD x 1.64 mm thick and 0.8 g – 5 pack
HSG-MEA-5C 6.4 mm ID x 8 mm OD x 0.5 mm thick; 0.05 g – 5 pack
HSG-MEA-5CD 6.4 mm ID x 8 mm OD x 1.0 mm thick and 0.1 g – 5 pack
HSG-MEA-5D 6.0 x 3.3 mm ID x 12.7 mm OD x 0.5 mm thick; 0.17 g – 5 pack- oval interior
HSG-MEA-5DD 6.0 x 3.3 mm ID x 12.7 mm OD x 0.8 mm thick and 0.35 g – 5 pack- oval interior
HSG-MEA-5E 4.8 x 2.5 mm ID x 9.5 mm OD x 0.6 mm thick; 0.4 g – 5 pack- oval interior
HSG-MEA-5ED 4.8 x 2.5 mm ID x 9.5 mm OD x 1.0 mm thick and 0.8 g – 5 pack- oval interior
HSG-MEA-5F 10.0 mm ID x 13.8 mm OD x 0.8 mm thick; 0.14 g – 5 pack – easy pick and place
HSG-MEA5-FD 10.0 mm ID x 13.8 mm OD x 1.5 mm thick and 0.31 g – 5 pack – easy pick and place
HSG-5G 9.5 mm ID x 12 mm OD x 0.5 mm thick; 0.2 g – 5 pack – polyester mesh with 800um square grids
HSG-5H 9.5 mm ID x 12 mm OD x 0.5 mm thick; 0.2 g – 5 pack – nylon mesh with 1 mm square grids.

Additional information;
These new Harp Slice Grid versions which are customized; Four new - Two offer superior perfusion flow. Anotheroffers ease of placement and pick up in a chamber. The last set offer a nylon mesh with 1mm2 cubes or 1.18mm2cubes.
The following versions are painted with a resistant epoxy coating:
HSG-5D and HSG-5DD -The HSG-5D versions have an oval interior for superior perfusion coverage.
HSG-MEA-5D and HSG-MEA-5DD-The HSG-MEA-5D versions have an oval interior for superior
perfusion coverage.
HSG-5E and HSG-5ED-The HSG-5E versions are similar to the HSG-5D versions but are smaller in size and weigh less.
HSG-MEA-5E and HSG-MEA-5ED­-The HSG-MEA-5E versions are similar to the HSG-MEA-5D versions but are smaller in size and weigh less
The 5F, 5G, & 5H versions are glass coated.