The MultiClamp™ 700B Microelectrode Amplifier from Molecular Devices is a computer-controlled microelectrode current- and voltage-clamp amplifier for electrophysiology and electrochemistry. This versatile amplifier is capable of singlechannel and whole-cell voltage patch-clamp, high-speed current-clamp (sharp electrode or field potentials), ion-selective electrode recording, amperometry/voltammetry and bilayer recordings. The MultiClamp 700B amplifier is designed to support up to two primary CV-7B headstages and two optional auxiliary (HS-2 or VG-2 type) headstages. Each CV-7B headstage contains a currentto-voltage converter for voltage-clamp and a voltage follower for true current-clamp. This allows the user to conveniently switch between low-noise patch-clamp recording and high-speed current-clamp recording. An optional CV-7B/BL headstage is available for bilayer recording.
The MultiClamp 700B Amplifier uses either an external trigger command or userprogrammable voltage threshold for rapid, automatic mode-switching between current and voltage-clamp. For example, by presetting a voltage threshold in current-clamp mode, the amplifier will automatically switch to voltage-clamp mode when the membrane potential reaches the threshold voltage. A user-specified delay can be programmed allowing further customization of recording procedures.
Sudden changes in membrane or pipette parameters may result in undesirable oscillations during whole-cell recordings. To overcome this, the MultiClamp 700B amplifier detects current or voltage oscillations and automatically disables or intelligently reduces compensation settings to protect the cell from damage.
Slight voltage drift, often due to changing electrode properties, may contaminate an otherwise decent current-clamp recording. In order to maintain the membrane potential at a consistent level, the MultiClamp 700B amplifier automatically injects a compensatory current over a user-defined time course.
The MultiClamp 700B amplifier enables researchers to perform experiments that were previously not possible using a single amplifier.
Traditionally, amplifiers are designed for optimal performance in voltage-clamp or current-clamp mode, but cannot perform both during the same experiment. The CV-7B headstages supplied with the MultiClamp 700B amplifier overcome this limitation by integrating both current-to-voltage and voltage-following circuitry. This design allows users to rapidly switch between patch-clamp recording and high-speed current-clamp recording. The CV-7B headstage has four different feedback resistors in voltage-clamp mode, allowing for a wide range of cellular recording. In current-clamp mode, the CV-7B headstage provides three different current setting resistors to clamp current from a few pA up to 200 nA.
With two headstages, the MultiClamp 700B amplifier can perform the function of two patch-clamp, two current-clamp, or a combination of patch- and current-clamp amplifiers. In addition, two optional voltagefollower headstages (HS-2 type) can be connected to auxiliary inputs to allow third- and fourth-point voltage recording. Dual headstages allow more complex synaptic experiments to be performed, as well as increasing throughput for drug discovery experiments, all at a significantly lower cost per channel.
Specialized headstages are available for bilayer and electrochemistry recordings. The optional CV-7B/BL headstage was designed to handle the large membrane capacitances found in bilayer recording. The CV-7B/EC headstage was designed to handle the large (±2V) command voltages required during electrochemistry recording.
The small profile of the CV-7B headstage makes it easy to incorporate into an electrophysiology setup. The dovetail design integrates with a base plate for easy attachment to micromanipulators. The CV-7B headstage contains both resistor feedback voltage-following circuitry for true current-clamp operation, and voltage-to-current circuitry for voltage-clamp operation.
The MultiClamp 700B amplifier is fully controlled by the MultiClamp Commander software. Computer control allows for tremendous flexibility, including broad ranges of current passing and recording levels, extensive filtering options and multiple signal outputs.
Computer control simplifies the patching process by providing automation of pipette offset, fast/slow electrode capacitance compensation, whole-cell capacitance compensation, series resistance correction, pipette capacitance neutralization and bridge balance - all without moving parts.
As an alternative to keyboard/mouse control, a separate control panel – the SoftPanel – is available (not included) as an option to provide manual knobs and buttons that replicate the key functions in the MultiClamp Commander.
What is included: 1) Two 1-CV-7B headstages, 2) Power cord, 3) USB cable, 4) Two Electrode Holders (Part number 1-HL-U), Patch-1U Model Cell (Part number 1-PATCH-1U), and CD with MultiClamp Commander software & Manual
NeuroLabParts provided us with great quality second hand Patch clamp equipment. The prices were very affordable and the items were almost brand new. Their customer service, maintenance and warranty services were GREAT and they helped us a lot in setting up our lab.
They would even follow up with us for updates on the performance of equipment and are always ......
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