BTX ELECTROCELL MANIPULATOR MODEL:BT600 Rev G

Descriptions:

This ECM-600 Electro Cell Manipulator is in great condition and has been tested to perform to factory specifications. This unit is a highly flexible electro cell manipulator and will have the capacity to perform most rapid transformations without the need for additional equipment.Important Notice: Other accessories, manuals, cables, calibration data, software, etc. are not included with this equipment unless listed in the above stock item description.

BTX ECM600 Electro Cell Manipulator
The BTX ECM600 Electroporation System is a general purpose Electro Cell Manipulator providing the most extensive features available in a single unit design. Only BT600 offers such great instrument flexibility and ease of use. All of the necessary power and capacitance for rapid and efficient transformation of bacteria, microorganisms, plat and mammalian cells is contained in the ECM600 without the needs for additional equipment

Features:

• Low and High Voltage Modes
• Resistance and Capacitance Timing
• Automatic Charge and Pulse
• Capacitance Range of 25-3175 µF
• Voltage Range of 50-2500 V Peak
• Power requirements 100-120VAC,350W,50-60Hz

Electro Cell Manipulator 600
Methods for Rapid Verification of Electroporation in Various Cell Types
1) Dictyostelium discoideum (slime mold)
Cells, in particular growth and developmental phases, were washed twice in ice-cold Bonner's tandard salt solution (BSS), and once in 50 mM sucrose. An aliquot (250 - 300 μl) of cell suspension inthe presence of 50 μg/ml FITC dissolved in 50 mM sucrose was transferred into an ice-cold chamber eqipped with two flat stainless steel electrodes that were separated at a distance of 2 mm.
Electroporation and subsequent washings by centrifugation were performed cold, and the samples
observed under a fluorescence microscope to determine the ratio of cells with FITC to total cells.
Incidentally, FITC was barely incorporated into the cells without electroporation.

2) Saccharomyces spp. (yeasts)
Yeast were grown in YPD medium, harvested while in exponential phase and resuspended in aminimal medium (50 mM Na + 50 mM K phosphate buffer, pH 6.8, 1 mM glucose, 5 mM NH4CI) and0.1 mM FITC dextran (green fluorescence, 0.01 mol FITC per mol glucose). Control aliquots wereremoved before and after the electrical pulse experiments, corresponding to exposure of 5 and 40minutes to the FITC-dextran. These initial and final controls are used to determine the spontaneousuptake and adsorption, and also loss of viability which is unrelated to electroporation. For each electric field strength, E, a single Aliquot (14-25 μl; 6 x 107cells/ml) of cell suspension was used to fill a parallel plane (stainless-steel electrodes) electroporation chamber at room temperature (23 ±3oC). Cells were then placed in a micro-centrifuge tube, held for a 5 min uptake/recovery period, centrifuged at 5000-7500 x g for 3 min, and washed twice with a minimal medium. 

3) Fusarium oxysporum (fungi macroconidia)
Macroconidia were suspended in water containing various concentrations (50 - 150 mg/ml) of FITCalbumin incubated at 28oC for 1 - 5 hr, and subjected to electroporation. Macroconidia were collectedby centrifugation, washed 3 times with water, and observed with an Olympus fluorescence microscope.For spore germination, electroporated spores were washed with water, suspended in Czapek-Doxmedium, and incubated statically at 28oC for two weeks. Sample solutions were sterilized bymembrane filtration and the reaction chamber was autoclaved before experiment. All of theexperiments were carried out under a stream of sterile air.

4) P3X63Ag8, CV-1, HeLa, Mouse Kidney Cells (MKC) mammalian)
The P3X63Ag8 cells and trypsinized CGV-1, HeLa or secondary MKCs were centrifuged at 250 X g,resuspended and washed in the appropriate electroporation buffer by recentrifugation. FITC-dextran of SFV-C protein were added to the cell suspension before electroporation. The electroporation chamber was cooled to 0oC, and filled with cell suspension (105 cells). Thereafter, the cells were kept for 10 min at 0oC (diffusion loading time). They were then subjected to a short centrifugation (5s,reaching 1500 rpm at the end of acceleration) before they were suspended in the appropriate resealing buffer or growth medium. Fluorescence microscopy was performed with a Nikon fluorescence microscope.

Applications:

• Gene Editing and Transfection: Introducing genetic material into cells for research or therapeutic purposes.
• Stem Cell Research and Regenerative Medicine: Modifying stem cells or reprogramming them for therapeutic applications.
• Gene Therapy: Delivering therapeutic genes for treating genetic diseases or disorders.
• Cancer Research and Immunotherapy: Transfecting immune cells for cancer treatment, including CAR-T therapy.
• Vaccine Development: Electroporating DNA or mRNA for antigen delivery.
• Protein Expression Systems: Producing recombinant proteins for research, therapeutic, or industrial applications.
• Drug Delivery and Toxicology: Delivering drugs or small molecules for testing or therapeutic purposes.
• Microbial Transformation: Introducing genes into bacteria or yeast for genetic studies or biotechnological applications.
• Viral Research and Production: Producing viral vectors or conducting viral research for gene delivery.
• In Vivo Electroporation: Delivering genes directly into tissues or organs for experimental or therapeutic purposes.