The ez-gSEAL 100B pressure controller is primarily designed for automated patch clamping, but its versatility also allows for various other applications. With just a simple click of a button, the ez-gSEAL pressure controller makes patch clamping effortless. It can be used in conjunction with our ezPATCH 100A smart manipulator, PatchMAX 100B automated patch clamp system, ChannelMAX 100A Mini automated dual patch clamp system or ChannelMAX 100A Twin automated quad patch clamp system for fully automated patch clamp experiments. Alternatively, it can be used as a stand-alone product on a traditional rig. Moreover, the controller can be utilized for drug puffing or studying stretch channels. The software-controlled pressure controller comes equipped with pumps, thereby eliminating the need for air tanks.
The ez-gSEAL 100B pressure controller boasts several enhancements compared to its predecessor, the 100A model. The pumps are now more robust and have an extended lifespan. Furthermore, the air flow within the unit has been optimized, leading to superior performance. Additionally, the unit now includes a pressure gauge that assists users in determining the ideal pressure for making seals and breaking in.
Software Control
The NBSController, which is the ez-gSEAL control software, regulates pressure application and measures pressure. It conveniently enables users to preset commonly-used pressures, simplifying the process of making seals and breaking in. With just a few clicks, users can achieve their desired outcomes. iPod Touch Control
The ez-gSEAL pressure controller is compatible with iPod Touch, iPhone, or iPad. By connecting to the server software, the NBSController, through WiFi, users can utilize their iPod Touch as a remote control to operate the device from their setup. This feature provides users with the flexibility to control the pressure controller at a distance.
Patch Clamp
With the ez-gSEAL pressure controller, patch clamping experiments become remarkably simple, requiring only a few clicks of a button. To initiate the process, users click the first button to set the positive pressure, followed by the second button to set the negative pressure required for making seals, and the third button to set the holding pressure. Breaking into the cell is equally straightforward, requiring users to click a single button to apply pulses. Thanks to its intuitive design, the ez-gSEAL pressure controller streamlines complicated patch clamp experiments, making them significantly easier to perform.
The measurement function of the ez-gSEAL control software enables users to accurately measure the pressure required for making seals and breaking in. Additionally, a wide range of cells and tissues, including HEK293 cells, brain slices, and oocytes, have been tested, and the seal parameters are readily available for these types of samples. New users can conveniently leverage these parameters to make seals and break in during their first attempt. To obtain available seal parameters, please contact NeoBiosystems.
With the incorporation of a DAQ board from National Instruments and our BNC interface box, the process of making seals and breaking in can be completed with just a single click of a button. This streamlined approach significantly simplifies the entire process, allowing researchers to perform their experiments with ease and accuracy.
When used in conjunction with the ezPATCH 100A manipulator, patch clamping becomes even simpler. All that is required is to aim the patch pipette at the cell that requires patching. Once the button is clicked, the pipette will touch the cell, creating a seal or even achieving whole-cell configuration automatically. This intuitive approach minimizes user error and streamlines the patch clamping process for optimal efficiency.
System Specifications of ez-gSEAL 100B
- Pressure range -250 mmHg to 250 mmHg
- Pressure resolution +/- 1.5 mmHg
- Minimal pulse duration 7 ms
- Minimal pulse interval 7 ms
- Maximum flow rate 33 sclm
- Filtration 40 micron
- Computer interface- USB
- Line voltage:110 VAC to 240 VAC
Specifications of ezPATCH 100A
-Major Components
- One micro-manipulator, which includes a motorized linear stage and a manual XY stage
- One PCI data acquisition board with BNC interface box
- Control software NBSController version 3.0
- Line voltage: 100 VAC to 120 VAC, or 220 VAC to 240 VAC
-Motorized Stage
- Travel: 28 mm
- Resolution: 0.05 µm
- Maximum speed: 4.8 mm/s
- Lowest speed: 0.5 µm/s
- Serial/USB interface
-Manual XY Stage
- Modular ball bearing linear stages
- Sensitivity 0.75 µm
- One inch travel
- Drift less than 1 µm
-Data Acquisition Board
- Sixteen analog Inputs, 16-bit, 250 kS/s, Input Impedance: > 10 GΩ in parallel with 100pF
- Two 16-bit analog outputs, 740 kS/s per channel. Output impedance: 0.2 Ω
- 24 digital I/O lines, 32-bit counters; digital triggering
- Analog input range: ± 10 V
- Analog output range: ± 10 V
The BNC interface box comes with 10 Analog INs and 2 Analog OUTs. A trigger is provided for sync in case needed. For the best grounding result, software can control the BNC interface box to be either in float or grounded condition.
Follow us