FLUXION IonFlux 16 automated patch clamp system

Fluxion IonFlux 16 Automated Patch Clamp System (Used, Tested, Fully Operational)

You are purchasing a used Fluxion IonFlux 16 Automated Patch Clamp System, a state-of-the-art platform designed for high-throughput electrophysiology. This system is ideal for ion channel research, drug discovery, and assay development, offering 16 parallel amplifier channels for simultaneous recordings. With advanced microfluidic plates and superior fluidic control, the IonFlux 16 stands out for its ability to handle complex assays that are difficult or impractical with traditional patch clamp systems.

✅ Condition:Used, fully operational, and in good working order.

✅ Guarantee: Fully functional and ready for use.

Key Features:

• 16 Parallel Channels: The IonFlux 16 system allows for 16 independent amplifier channels running in parallel, enabling high-throughput ion channel experiments with precision and reliability.
• Advanced Microfluidic Plates: IonFlux is the only system where all cell recordings and liquid exchange occur directly within the plate, without the need for additional liquid handling equipment. This unique feature allows for:
• Rapid and parallel solution exchange for faster experimental results
• Continuous flow of extracellular solutions and compounds throughout the experiment, maintaining optimal conditions.
• Automated Patch Performance: Automated patch clamp technology provides the performance and throughput of a manual patch clamp rig, but without requiring specialized expertise. Perfect for researchers without patch clamp experience.
• High-Quality Recordings: Offers high-quality recordings from 16 independent amplifier channels, ensuring accurate and reproducible data for ion channel assays.
• Temperature Control with Feedback: Includes temperature control with positive feedback, maintaining a constant and optimal environment for cell recordings.
• Flexible Assays: IonFlux 16 is capable of conducting specialized assays such as:
• Ligand binding
• Allosteric modulation
• Multiple agonist studies
• Voltage- and temperature-sensitive assays
• Ideal for Ion Channel Research: Designed for applications such as functional expression of ion channels, mutant screening, drug profiling, and pharmacology.
• High-Throughput Solution: The IonFlux 16 is ideal for researchers requiring high-throughput screening of ion channel targets, while maintaining flexibility for a range of experimental setups.

Technical Specifications:

• Channels: 16 independent patch clamp amplifier channels running in parallel
• Fluidic System: Proprietary Well Plate Microfluidic™ technology for continuous solution flow and rapid compound addition
• Recording Mode: Extracellular recording with high precision and quality
• Temperature Control: Positive feedback for precise temperature regulation during experiments
• Amplifiers: High-performance amplifiers for clear and accurate data acquisition
• Assay Flexibility: Supports a wide range of assays, including ligand binding, allosteric modulation, multiple agonists, and voltage-sensitive assays
• Cell Ensemble Recording: Simultaneous recording from a population of cells for better reliability and reduced variability
• Data Throughput: The IonFlux 16 can produce up to 2,000 data points per day, enabling rapid data acquisition for high-throughput applications.

System performance

Daily throughput: 2000 data point

Number of simultaneous amplifier channels:16

Cell recording configuration; Ensemble recording

Compound application time: <100ms

Plate format

Plate type:96-well, SBS format

Total data points per plate:128

Unique compounds/concentrations per plate:64

Number of experimental patterns per plate: 8

System Specifications Common to IonFlux 16 and IonFlux HT

Instrument configuration

 Instrument dimensions: 20” (50 cm) X 20” (50cm) X 10” (25 cm)

Amplifier configuration: Sampling rate adjustable to 20 khz Capacitance, leak, series resistance compensation

Temperature control: Ambient to 40ºC

Cell recording format; 20 cells per ensemble recording, 2X redundancy

Software

Operation modules: Protocol editor, run table, assay development mode, recording visualization, analysis, and data export

Operating system: Windows 7, XP, Vista

External compatibility

 Liquid handling automation: Compatible with most liquid handlers, SDK available

 Multiplexing ability: Integrate up to 4 readers per liquid handler

The primary difference between the Fluxion IonFlux 16 and IonFlux HT automated patch clamp systems is the number of independent amplifier channels, with the IonFlux HT having significantly more, allowing for much higher throughput in high-throughput screening applications compared to the IonFlux 16 which is better suited for standard ion channel research and assay development.

Key points:

Channel count: IonFlux 16 has 16 amplifier channels, while IonFlux HT has 64 channels.

Application: IonFlux 16 is ideal for standard ion channel research and assay development, while IonFlux HT is designed for high-throughput screening of ion channel targets.

Throughput: Due to its higher channel count, IonFlux HT can process a much larger number of samples per run compared to IonFlux 16.

Included:

• Fluxion IonFlux 16 Automated Patch Clamp System
• Microfluidic Plates
• Power cord

IonFlux System

The Fluxion IonFlux system delivers an unparalleled solution for high-throughput ion channel screening, combining advanced patch clamp technology with proprietary microfluidic control. It offers a user-friendly, automated solution that simplifies complex assays without compromising on performance, making it ideal for drug screening, ion channel research, and assay development. The IonFlux 16 is specifically designed for individual research labs, providing a powerful tool for studying ion channel function, mutant screening, and drug profiling.

Applications:

• Ion Channel Research & Assay Development: Perfect for studying the function and pharmacology of ion channels, including both voltage-gated and ligand-gated channels.
• Drug Discovery: Utilized for drug screening, pharmacology, and cardiac safety profiling (including hERG testing).
• Functional Expression & Mutant Screening: Enables functional expression of ion channels and the screening of genetic mutants for specific pharmacological responses.
• Ligand-Gated & Voltage-Gated Studies: Facilitates ligand-gated and voltage-gated studies, providing a comprehensive platform for diverse ion channel assays.